The DEAD box RNA helicase DDX42 is an intrinsic inhibitor of positive-strand RNA virusesArticle Published on 2022-11-072022-11-16 Journal: EMBO Reports [Category] SARS, 변종, [키워드] Antiviral approach cell line Complexes CRISPR DDX42 DDX42 DEAD-box RNA helicase Depletion DNA dominant-negative elements expression Helicase HIV-1 HIV-1 infection identify immunoprecipitation in vitro increase Infection inhibit inhibitor Interaction intrinsic intrinsic immunity LINE-1 mutant overexpression Perspective primary cells Replication restrict reverse transcription ribonucleoprotein RNA RNA virus RNA viruses RNA-seq analysis SARS-CoV-2 target cells viral infections viral inhibition. viruses [DOI] 10.15252/embr.202154061 PMC 바로가기
Transcriptional regulation and small compound targeting of ACE2 in lung epithelial cellsArticle Published on 2022-11-012022-11-15 Journal: Acta pharmacologica Sinica [Category] COVID19(2023년), SARS, 진단, [키워드] 16HBE 16HBE cell 16HBE cells 6-O-angeloylplenolin ACE2 ACE2 expression addition administration Cell Centipeda Centipeda minima chemokine Chinese Compound conducted COVID-19 CX3CL1 CXCL13 dataset demonstrated downregulated entry of SARS-CoV-2 enzyme evaluated expression gene encoding HLF IL-6 inhibited inhibitor Interaction lung epithelial cell Lung epithelial cells lung tissue lung tissues mice modulate parental pathogen phosphorylated positively correlated promoter PROTECT Protein pseudovirus receptor reduced regulate Regulation S protein SARS-CoV-2 SARS-COV-2 infection screened STAT3 STAT3. stimulated suppressed target cells tested TFS Transcription transcription factor transfected with [DOI] 10.1038/s41401-022-00906-6 PMC 바로가기
Severe COVID-19 patients display hyper-activated NK cells and NK cell-platelet aggregatesArticle Published on 2022-10-052022-11-15 Journal: Frontiers in Immunology [Category] COVID19(2023년), SARS, 진단, 치료제, [키워드] absence activated Activation aggregate aggregates analysed antiviral immunity Blood blood cell CD107a CD62P Cell cells changes in chemokines Cohort correlated COVID-19 COVID-19 disease COVID-19 patient cytokine Cytokines Cytometry cytotoxicity discharged disease pathology early stage Ex vivo Features flow cytometry form Frequency gene signature hospital hospitalised hospitalised COVID-19 patient ICU ICU patient IFN-γ IL-15 in vitro increased expression Infection innate immune response intensive care unit killing majority natural Neutrophils NK cell NK cells Patient patients phenotype phenotyped plasma Platelet platelet aggregates. Platelets Post-COVID-19 patients quantified SARS-CoV-2 scRNA-seq severe COVID-19 disease severe symptoms Single-cell RNA-sequencing Stage subset Symptom Symptoms target cells the timing TNF-α viral infections were measured while [DOI] 10.3389/fimmu.2022.861251 PMC 바로가기
Association of ACE2 Gene Variants with the Severity of COVID-19 Disease-A Prospective Observational StudyArticle Published on 2022-10-022022-11-16 Journal: International Journal of Environmental Research an [Category] COVID19(2023년), SARS, 변종, [키워드] ACE2 ACE2 gene acute respiratory syndrome allele Angiotensin receptors angiotensin-converting enzyme-2 candidate caused coronavirus correlation COVID-19 COVID-19 disease COVID-19 pandemic disease evaluate gene polymorphism genetic susceptibility Hospitalization host cell host receptor identify increased risk intensive care Patient polymorphism Prospective recent risk Risk factors rs2285666 rs4646156 SARS-CoV-2 SARS-COV-2 infection severe disease course severity severity of COVID-19 single nucleotide single nucleotide polymorphism. statistically significant target cells the SARS-CoV-2 virus triggered variant virus [DOI] 10.3390/ijerph191912622 PMC 바로가기
Delivery of Antibody-Like Molecules, Monobodies, Capable of Binding with SARS-CoV-2 Virus Nucleocapsid Protein, into Target CellsArticle Published on 2022-10-012022-11-15 Journal: Doklady. Biochemistry and biophysics [Category] COVID19(2023년), SARS, 진단, [키워드] antibody-like molecules C-terminus cellular cellular thermal shift assay delivery demonstrated E. coli expressed fluorescent fused HEK293T cell highest affinity Interaction molecules monobody nucleocapsid nucleocapsid protein penetration peptide Previous studies produced Protein replacement resulting S10 shuttle peptide SARS-CoV-2 selected significantly target target cells TAT TAT peptide the SARS-CoV-2 thermophoresis. transfected with [DOI] 10.1134/S1607672922050088 PMC 바로가기
Monitoring fusion kinetics of viral and target cell membranes in living cells using a SARS-CoV-2 spike-protein-mediated membrane fusion assayArticle Published on 2022-09-262022-11-15 Journal: STAR protocols [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] acute respiratory syndrome approach cell membrane cell types Cell-based Assays Complete coronavirus functional living cell living cells membrane fusion Microbiology Microbiology. MONITOR monitoring pathogenicity Protein protocol reporter SARS-CoV-2 Spike protein Suzuki target cells triggering variants viral entry virus [DOI] 10.1016/j.xpro.2022.101773 PMC 바로가기
Could Endogenous Glucocorticoids Influence SARS-CoV-2 Infectivity?Article Published on 2022-09-212022-11-15 Journal: Cells [Category] SARS, 변종, 치료제, [키워드] ACE2 ACE2 (angiotensin converting enzyme 2) Affect alteration analogues Antiviral component conformational coronavirus coronavirus diseases cortisol COVID-19 Deleterious Dexamethasone endogenous enzyme glucocorticoid Glucocorticoids groups Host Immunological disorders Infection influence inhibit SARS-CoV-2 Innate immunity Interaction mechanism outcome pathogen produced PROTECT Protein SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 receptor Signal transduction spike Spike protein spike protein. stimulate subunit surface protein target cells variant [DOI] 10.3390/cells11192955 PMC 바로가기
SARS-CoV-2 requires acidic pH to infect cellsArticle Published on 2022-09-202022-11-15 Journal: Proceedings of the National Academy of Sciences of [Category] SARS, 치료기술, 치료제, [키워드] 3D imaging Acidic acidic pH acute respiratory syndrome cathepsin Cell cell entry cleavage coronavirus detect endosomal furin Genome infect cell Infection Infection route live-cell imaging membrane membrane fusion nasal occurred penetration producing SARS-CoV-2 target cells TMPRSS2 TMPRSS2-expressing cell virion virus entry Virus entry. [DOI] 10.1073/pnas.2209514119 PMC 바로가기
Receptor-Binding Domain (RBD) Antibodies Contribute More to SARS-CoV-2 Neutralization When Target Cells Express High Levels of ACE2Article Published on 2022-09-162022-11-15 Journal: Viruses [Category] SARS, 진단, [키워드] ACE2 ACE2 binding ACE2 expression Affect antibodies antibody Cell cell receptor domain Effectiveness expression Infection lentivirus Level monoclonal monoclonal antibody N-terminal domain neutralization Neutralization assay neutralize Neutralizing activity neutralizing antibody neutralizing antibody epitopes NTD polyclonal antibody polyclonal human sera pseudovirus Pseudovirus neutralization assays RBD Region SARS-CoV-2 Serum Neutralization spike epitope subset target target cell target cell receptor expression. target cells the RBD therapeutic [DOI] 10.3390/v14092061 PMC 바로가기
Association of the Transmembrane Serine Protease-2 (TMPRSS2) Polymorphisms with COVID-19Article Published on 2022-09-072022-11-15 Journal: Viruses [Category] SARS, 치료기술, 치료제, [키워드] ACE2 receptor anti-SARS-CoV-2 antibody cellular protease COVID-19 COVID-19 disease disease dominant exonuclease Genetic genetic susceptibility Genotype Haplotype high risk individual Infection lactic acid dehydrogenase LDH Patient patients with COVID-19 polymorphism Polymorphisms Protective risk risk of COVID-19 RT-PCR test SARS-CoV-2 SARS-COV-2 infection Serine TaqMan assays target cells the SARS-CoV-2 TMPRSS2 transmembrane transmembrane serine protease-2 (TMPRSS2). with COVID-19 [DOI] 10.3390/v14091976 PMC 바로가기